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. 2015 Dec 2;46(2):455–463. doi: 10.1002/eji.201545537

Figure 4.

Figure 4

TSLP secretion requires independent activation of ER stress and IL‐1‐dependent signaling. (A) Human mDCs were preincubated for 1 h with or without IRE1α or PERK inhibitors and were then stimulated with CUR, MP, or C. albicans for 24 h (n = 12 independent donors, presented as pooled data). IL‐1β was measured in 24‐h cell culture supernatants by ELISA. (B) Human mDCs were preincubated for 1 h with or without IRE1α or PERK inhibitors and were then stimulated with CUR, MP, or C. albicans with or without recombinant IL‐1β for 24 h (n = 9 independent donors, presented as pooled data). TSLP was measured in 24‐h cell culture supernatants by ELISA. Cumulative data are displayed as mean ±SEM. Statistical significance was calculated using one‐way ANOVA with Bonferroni post‐tests (***p = 0.001, **p = 0.01, *p = 0.05).