Figure 6.

LPS-activated transcription factors in macrophages bind to Flvcr promoter. Nuclear extracts were prepared from primary murine BMDM and incubated with or without purified Flvcr promoter DNA. Transcription factor (TF) activity was measured using the Signosis Promoter Binding Transcription Factor Profiling Array 1 and results are presented as the amount of TF activity without promoter over TF activity with promoter, reflecting promoter binding. The dotted line indicates 1.5-fold change. Several of the TF with highest Flvcr promoter binding (STAT4, AP2, SP-1, and IRF-1) had predicted binding sites with the human Flvcr promoter (Supplemental Figure 1), and STAT4 and IRF-1 are known downstream mediators of LPS/TLR4 signaling in macrophages. The mean and SEM are shown (n = 3 mice).