Skip to main content
. 2016 Mar 9;6:27. doi: 10.3389/fcimb.2016.00027

Figure 2.

Figure 2

Expression of miRNAs and their predicted targets at 15, 30 min, and 4 h in viable and heat killed (HK) mycobacterial infection to macrophages. (A) The miRNAs, miR-3619-5p and miR-324-3p were down-regulated in all time points whereas miR-637 was down-regulated only at initial period of time (15 min) in both viable and HK bacterial infections. (B) The predicted targets, CTSS and RAB11FIP4 were up-regulated in later period of time points (4 h) in both viable and HK bacterial infections. (C) Expression of CTSS in THP-1 cells was down-regulated after transfection with miR-3619-5p mimics. (D) Three different sites were found after screening the 3′ UTR region of CTSS for potential miR-3619-5p binding sites using RNAhybrid. (E) The interaction between miR-3619-5p and human CTSS was verified by means of reporter gene assays using HeLa cells. Identified target sites between miR-3619-5p and CTSS were analyzed using RNAhybrid. Relative luciferase activity (Luc Gaussia: LucCypridina) was determined respective to nonsense miRNA mimics. The columns show means of three biological replicates each measured in triplicates while error bars show the standard deviation. Asterisks indicate statistical significance between samples (*P < 0.05).