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. 2016 Mar 9;7:90. doi: 10.3389/fimmu.2016.00090

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Copyright © 2016 Jansen, van Herwijnen, van Kooten, Hoek, van der Zee, van Eden and Broere.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Antigen recognition by mB29b-TCR transgenic mouse. PBLs from two founders, positive for the TCRα and TCRβ chain, were cultured for 96 h with irradiated A20 B cells and stimulated with 2 or 20 μg/ml B29, or 5 μg/ml ConA as a positive control. PBLs from founder 2 proliferated in a dose-dependent response. The PBLs from founder 1 did not show any proliferation after B29 stimulation. The PBLs from both founders produced IL-2 but the PBLs from founder 2 produced higher amounts. Proliferation was determined by ³H-thymidine incorporation during the final 16 h of culture and IL-2 production was determined by Luminex. Data are from one experiment.