Figure 3.

Fluorescence microscopic images of HEK-293T cells previously incubated with adenoviral vector that had undergone disinfectant exposure. Adenovirus vector was applied onto cells after 1-min contact times in suspension with disinfectants A through D diluted with FBS to 75%, 50%, or 25% of the recommended concentration. GFP expression increases with decreasing disinfectant concentration. Microscopy is less sensitive than flow cytometry in detecting fluorescence. Magnification, 10× (initial images); bar, 100 µm.