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. 2015 Sep 25;65(1):45–51. doi: 10.1538/expanim.15-0038

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©2016 Japanese Association for Laboratory Animal Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.

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Fig. 2. — Effects of valproic acid on histone H3 acetylation. (A) Adipose tissue-derived stem cells (ASCs) from rats were incubated with valproic acid (VPA) or valpromide (VPM), an analogue of VPA without histone deacetylase inhibitory activity, for 3 days. Control group was treated with solvent, DMSO. ASCs were immunostained with an anti-acetylated histone H3 (K9) antibody (Red). Acetylated histone H3 (K9) antibody was visualized with Cy3-conjugated goat anti-rabbit IgG. Scale bar, 100 μm. (B) VPA increased acetylation levels at the proximal region of TUBB3. The state of the histone tails of ASCs pretreated with VPA for 3 days was analyzed by ChIP assay using antibody to acetylated lysine 9 on histone H3 (anti-AcH3K9). Typical electrophoresis of PCR analysis of TUBB3 was shown.