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. 2016 Mar 9;6:22273. doi: 10.1038/srep22273

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Copyright © 2016, Macmillan Publishers Limited

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Hep 3B2.1–7 (A,B) and HuH-7 (C,D) hepatoma cells were grown to ~95% confluency in monolayer and straight line scratches were made across each monolayer (19). Cells were incubated with 0 (control), 1, and 5 μM PD 169316 (A,C) or SB 525334 (B,D). The exact scratch areas were recorded immediately and after two days. Invaded areas were quantified by ImageJ software. 8 Scratches were assayed in each monolayer and experiments were performed in duplicate. Error bars represent standard deviations.