Figure 4. Aneuploidy increases invasiveness of CRC cells.
The invasive capacity of the three different cell lines was assessed using a matrigel invasion assay. (A–D) Examples of Giemsa-stained invasive DLD1, DLD1 + 7, and DLD1 + 13 cells cultured under different conditions. Scale bar, 100 μm. (E–H) Quantification of invasive DLD1, DLD1 + 7, and DLD1 + 13 cells cultured under different conditions. The data are reported as mean and s.e.m. from three biological replicates. Statistical analysis showed that significantly larger numbers of aneuploid compared to diploid cells migrated through the matrigel layer (t-test, p < 10−4 for each aneuploid CRC cell line compared to diploid cells under all culture conditions).