Table 2. Kinetic parameters of reported artificial substrates of NSP4.
| No | Sequence | kcat [s−1] | KM [μM] | kcat/KM M−1 × s−1 | Ref. |
|---|---|---|---|---|---|
| 1 | MCA-PEG-Ile-Arg-Arg-Ser-Ser-Tyr-Ser-Phe-Lys(Dnp)-Lys | 0.136 ± 0.008 | 13.8 ± 3.3 | 10,000 | 18 |
| 2 | Ac-hCha-Phe(4-guanidine)-Oic-Arg-ACC | 2.0 ± 0.5 | 61.0 ± 9.1 | 32,786 | 19 |
| 3 | ABZ-Dap(O2(Cbz))-Dap(Cbz)-Dap(GO1)-Arg-ANB-NH2 | 1.0 ± 0.1 | 7.6 ± 0.5 | 131,579 | |
| 4 | MCA-Gly-Phe-Lys-Pro-Arg-Ser-Arg-Pro-Lys(Dnp)-arg-arg | ND | ND | ND | 16 |
Where Oic is octahydroindole-2-carboxylic acid, Phe(4-guanidine) 4-gaunidyl-l- phenylalanine, ACC 7-amino-4-carbamoylmethylcoumarine, Dnp 2,4-dinitrophenyl, MCA (7-methoxycoumarin-4-yl)acetyl acid, hCha cyclohexylo-l-homoalanine, arg = D-arg and PEG -8- amino)-3,6-dioxaoctanoic acid.