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. 2016 Mar 9;6:22849. doi: 10.1038/srep22849

Figure 3. Evaluation of the metabolic activity of mammalian cells cultivated in the presence of silver.

Figure 3

(a) L929 cells, primary osteoblasts and HUVEC were cultivated on different implants, while the metabolic activity was measured 24 h after seeding. The metabolic activity of cells grown on tissue culture-treated polystyrene (Co) was normalised to 100%, background levels of absorbance were subtracted. The silver content of the DLC is indicated in percentage. (b) Dose-dependent effect of silver nitrate (concentrations range from 0.1 to 1000 μM AgNO3) on the metabolism of L929, THP-1, SAOS-2 and HUVEC. Metabolic activity of non-treated cells was normalised to 100%, background levels of absorbance were subtracted. Data are represented as mean ± SD of at least 3 independent experiments. Statistical significant differences (P < 0.01) to the control group (co) are indicated by an asterisk. Ti = Ti6Al4V alloy; R = Ti6Al4V alloy with diamond-like carbon (DLC); B = DLC with 4.5 ± 0.5% silver; C = DLC with 1.7 ± 0.4% silver; D = DLC with 0.9 ± 0.2% silver.