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. 2016 Mar 9;6:22849. doi: 10.1038/srep22849

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(a) Silver release per area over ten days in simulated body fluid. (b) Metabolic activity of L929 cells cultivated on indicated specimens. The white bar reflects the metabolic activity of L929 cultivated on implants rinsed with water prior to the experiment. The metabolic activity of cells grown on tissue culture-treated polystyrene (co) was normalized to 100%, background levels of absorbance were subtracted. Data are represented as mean ± SD of at least 3 independent experiments. Statistical significant difference (P < 0.01) between the pretreated (B*) and the non-pretreated (B) specimens is indicated by the asterisk. Ti = Ti6Al4V alloy; R = Ti6Al4V alloy with diamond-like carbon (DLC); B = DLC with 4.5 ± 0.5% silver; C = DLC with 1.7 ± 0.4% silver; D = DLC with 0.9 ± 0.2% silver.