A–O. A–E. Lyve-1 staining (red) showing lymphatic vessels
(white arrows 40x), enlarged in the inset (63x, panel D). F–J. PNAd
staining (red), and K–O MAdCAM-1 staining (red) showing HEVs (white
arrows, 40x), enlarged in the inset (63x panels I and N). Note their
“plump” appearance, compared to panel D, typical of HEVs. Lymphocytes are
present in and around the HEVs (J, O). Each marker was visualized on separate
sections of the same TLT aggregate to avoid artifacts due to possible cross-reactivity
between secondary antibodies.