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. Author manuscript; available in PMC: 2016 Mar 9.
Published in final edited form as: Nature. 2015 Jun 17;522(7557):444–449. doi: 10.1038/nature14508

Figure 4. SF3B1 is required for pathologic cardiac growth and function.

Figure 4

a, Schematic representation of the AAV9-fl/fl-shSf3b1 virus before and after Cre-mediated recombination (left panel) and of the experimental timeline (right panel). b–d, Left-ventricular weight normalized to tibia length (LV/TL) (b), isovolumic relaxation time (IVRT) (c) and ventricular fructose levels (d) of sham- or 1K1C-treated Mlc2v-cre− and Mlc2v-cre+ mice injected with AAV9-fl/fl-shSf3b1#1 viruses. e, Immunoblots of heart lysates from Mlc2v-cre− and Mlc2v-cre+ mice operated and transduced as in b–d using indicated antibodies. f, g, LV/TL (f) and ejection fraction (EF) (g) of sham- or TAC-treated Mlc2v-cre− and Mlc2v-cre+ mice injected as indicated. h, Lysates of hearts from mice transduced and operated as in f were processed for immunoblotting with antibodies against denoted proteins. For a–d, f, g, number of mice per group is given in Extended Data Table 1. Error bars are s.e.m. *P < 0.05; **P < 0.01; two-tailed unpaired t-test (b–d, f, g).