Extended Data Table 2. Echocardiographic analysis of sham- or TAC-operated Khk-A^+/+/Khk-A^−/− and Khk-A/C^+/+/Khk-A/C^−/− mice.

	Khk-A+/+		Khk-A−/−
	sham	TAC	sham	TAC
n	6	6	5	6
BW (g)	25.6 ± 0.7	27.3 ± 0.8	25.4 ± 0.7	25.6 ± 0.7
IVS;d (mm)	0.73 ± 0.01	0.98 ± 0.02***	0.72 ± 0.01	1.00 ± 0.03***
IVS;s (mm)	1.03 ± 0.07	1.34 ± 0.04**	1.12 ± 0.01	1.36 ± 0.06**
LVID;d (mm)	3.85 ± 0.08	3.80 ± 0.06	3.75 ± 0.10	3.73 ± 0.07
LVID;s (mm)	2.71 ± 0.16	2.65 ± 0.11	2.66 ± 0.20	2.70 ± 0.10
LVPW;d (mm)	0.72 ± 0.01	0.99 ± 0.04***	0.72 ± 0.01	0.99 ± 0.03***
LVPW;s (mm)	1.04 ± 0.03	1.35 ± 0.03***	1.05 ± 0.01	1.32 ± 0.05***
%FS	34.0 ±2.1	26.3 ±1.5*	34.3 ± 1.7	26.0 ± 1.2*
E/A	1.38 ± 0.11	2.20 ± 0.05**	1.97 ± 0.4	1.90 ± 0.4
Aortic Vel. (mm/s)	938 ± 55	4250± 275***	880 ± 68	3700± 308***
	Khk-A/C+/+		Khk-A/C−/−
	sham	TAC	sham	TAC
n	5	6	6	5
BW (g)	25.8 ± 0.6	24.9 ± 0.8	24.51 ± 0.6	24.4 ± 0.6
IVS;d (mm)	0.72 ± 0.01	1.00 ± 0.05**	0.64 ± 0.04	0.83 ± 0.06
IVS;s (mm)	1.15 ± 0.01	1.42 ± 0.07*	1.06 ± 0.07	1.19 ± 0.13
LVID;d (mm)	3.53 ± 0.13	3.70 ± 0.23	3.65 ± 0.13	3.67 ± 0.04
LVID;s (mm)	2.28 ± 0.27	2.61 ± 0.33	2.49 ± 0.17	2.45 ± 0.10
LVPW;d (mm)	0.69 ± 0.03	1.00 ± 0.05***	0.67 ± 0.02	0.81 ± 0.04*
LVPW;s (mm)	1.12 ± 0.03	1.25 ± 0.03*	1.00 ± 0.06	1.11 ± 0.07
%FS	35.5 ± 3.0	27.5 ±1.6*	32.1 ± 2.30	33.4 ± 2.9
E/A	1.51 ± 0.09	1.56 ± 0.17	1.77 ± 0.10	1.64 ± 0.21
Aortic Vel. (mm/s)	928 ± 120	4020± 232***	937 ± 59	3600± 292***

BW, body weight; IVS;d, interventricular septum diameter in diastole, IVS;s, interventricular septum diameter in systole; LVID;d, left-ventricular inner diameter in diastole; LVID;s, left-ventricular inner diameter in systole; LVPW;d, left-ventricular posterior wall diameter in diastole, LVPW;s, left-ventricular posterior wall diameter in systole; %FS, fractional shortening; E/A, ratio of early (E) to late (A) ventricular filling velocities; Aortic Vel. = aortic velocity. Values shown are mean ± s.e.m.;

^*P < 0.05;

^**P < 0.01;

^***P < 0.001; two-tailed unpaired t-test.