Skip to main content
. 2016 Jan 27;17(2):162. doi: 10.3390/ijms17020162

Figure 4.

Figure 4

PROK1-V67I and -WT (A) enhanced cell invasion ability and (B) altered intracellular calcium influx in a dose-dependent manner. PROKR2-transfected cells had increased abilities of inducing calcium release and cell invasiveness compared with PROKR1-transfected cells. HEK 293 and HTR-8/SV neo cells were transiently transfected with control vector, WT or variant PROK1 construct. Photographs and quantification of invaded cells stained with Giemsa’s azur eosin methylene blue solution are shown in the upper and middle panels in A. HEK293 and HTR-8/SV neo cells were transiently transfected with either PROKR1 or PROKR2 plasmid and treated with various concentrations of PROK1 WT or V67I condition medium (0, 1.0, 2.5, 5.0 nM). Invaded cells (A, lower panel) and intracellular cell influx alternation (B, upper and lower panels) were measured. * p < 0.05; ** p < 0.01; *** p < 0.001 compared between PROKR1- and PROKR2-transfected cells, and groups of different concentration. PROK1 recombinant protein (Biovision, Milpitas, CA, USA) was used as a standard control to validate protein function of PROK1 (WT) and PROK1-V67I in condition medium (CM). N.S.: no significant difference.