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. 2016 Jan 28;17(2):169. doi: 10.3390/ijms17020169

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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ICT inhibited macrophage accumulation and MMP-1 protein expression, and up-regulated collagen protein content in intima of aorta. (A) Representative images of immunohistochemistry for macrophages CD68, MMP-1, SMC α-actin and Collagen type I in cross sections of aorta from control, HC, and HC+ICT groups. Scale bar = 200 µm. Black arrow: smooth muscle cells (third panel) and collagen type I (fourth panel); (B) Quantitative analysis of intimal macrophages-, MMP-1-, SMC α-actin- and Collagen type I-positive area reported as percentage of the entire intima area. * p < 0.05, between control and HC groups; ^# p < 0.05, between HC and HC + ICT groups. n = 9. Data were means ± SEM.