Skip to main content
. 2016 Jan 29;17(2):178. doi: 10.3390/ijms17020178

Figure 6.

Figure 6

Modulation of connexin 43 expression affects GJIC. (A) Gap junction activity of FMC2u determined by scrape load dye transfer after transfection with empty vector (control), GFP-Cx43 cDNA vector, or siRNA Cx43. A graphical representation of dye transfer in µm was shown next to the images. Red lines indicate a cross sectional cut. Lucifer yellow was used as a gap junctional dye and Rhodamine-dextran used to mark the cut site. Green fluorescence indicates the passage of dye form the cutting site. Images taken at 4×, 10×, and 20×. Scale bar = 100, 100, 50 µm, respectively. Graphical representation of ZO-1 expression in Cx43-immunoprecipitated FMC2u cells at 0, 6, 12, 24, 36, and 48 h post transfection; (B) Induced overexpression of Cx43 and (C) a silencing of Cx43. All experiments conducted with a sample size of n = 3. * p-value < 0.05 compared to respective transfection control.