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. 2016 Jan 29;17(2):182. doi: 10.3390/ijms17020182

Figure 3.

Figure 3

Effect of miR-30-5p on differentiation of C2C12 myoblasts. (A) Constructs and expression of miR-30-5p. RT-qPCR detection of mature miR-30-5p, using RNA prepared from C2C12 cells transfected with the expression constructs of miR-30-5p during the differentiation, confirming proper processing of miR-30-5p. The cells transfected with pcDNA3.1(+) as the control; (B) Western blot detection for MHC and MyoG proteins in the differentiated C2C12 cells respectively transfected miR-30a-5p, miR-30b-5p and miR-30e-5p constructs for six days in differentiation medium (DM). β-Tubulin was used as the loading control; (C) The C2C12 cells cultivated in DM (magnification 10×); (D) The expression levels of MHC and MyoG in C2C12 cells co-transfected with equivalent amount of miR-30a-5p, miR-30b-5p and miR-30e-5p were detected by RT-qPCR at 6 days after transfection in DM. control represents the C2C12 cells not transfected by miR-30-5p. Asterisks indicate significant differences. * p < 0.05; Error bars indicate SD (n = 3). Days (d) indicate the time the cells were in the differentiation medium. The expression level was normalized to GAPDH.