Figure 1.

Atrogin-1 is conserved between humans, mice, and zebrafish and zebrafish Atrogin-1 is expressed in the embryonic skeletal muscle and heart. (A) Protein alignment of Danio rerio (dr), Mus musculus (mm) and Homo sapiens (hs) Atrogin-1, identical amino acids are shaded in black, similar amino acids in grey. Conserved F-box domain is depicted in red; (B,B’) Immunofluorescent staining against Atrogin-1 on paraffin sections of zebrafish skeletal muscle at 72 hpf, nuclei are counterstained with DAPI, scale bar = 25 µm; (C-C’’’) Immunofluorescent staining against Atrogin-1 (green) and ß-Catenin (red) on dissected zebrafish hearts at 72 hpf, nuclei are counterstained with DAPI, scale bar = 10 µm.