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. 2016 Feb 5;17(2):213. doi: 10.3390/ijms17020213

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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SDS-PAGE of final preparations of recombinant CYP enzymes. Two-hundred pmol of CYP1A1 (lane 1), CYP1A1-S122A (lane 2), CYP1A2 (lane 3), CYP1A2-T124V (lane 4), CYP1B1 (lane 5) and CYP1B1-A133S (lane 6) was loaded onto gradient 4%–20% gel. The arrow indicates a protein band having a molecular mass of ~50 kDa.