Table 3.

Primers used for CYP gene modifications.

1A1	Correction of I171L
	Forward GAGGCTGAGGTCCTGATAAGCACGTTGCAGG
	Reverse CCTGCAACGTGCTTATCAGGACCTCAGCCTC
1a1	Histidine tag modification
	Forward GAATTCATATGGCTTTTCCAATTTCAATG
	Reverse TATCTAAGCTTCATTAATGATGATGATGATGATGAGAGCGCAGCTGCATTTG
1A1	S122A mutation
	Forward GTAATGGTCAGAGCATGGCCTTCAGCCCAGACTC
	Reverse GAGTCTGGGCTGAAGGCCATGCTCTGACCATTAC
1A2	Histidine tag modification
	Forward GAATTCCATATGGCTCTGTTATTAGCAG
	Reverse TATCTAAGCTTCATTAATGATGATGATGATGATGATTGATGGAGAAGCGCAG
1A2	T124V mutation
	Forward CTGGCCAGAGCTTGGTCTTCAGCACAGACTCTG
	Reverse CAGAGTCTGTGCTGAAGACCAAGCTCTGGCCAG
1B1	N-terminal and histidine tag modification
	Forward GACGAATTCATATGCTTTCTCCAAATGATCCATGGCCGCTAAACCCG
	Reverse GCCAAGGAAACTTGCCAACATCATCATCATCATCATTAATGAAGCTTAGATA
1B1	Correction of L432V
	Forward CTGTGAATCATGACCCACTGAAGTGGCCTAACCCG
	Reverse CGGGTTAGGCCACTTCAGTGGGTCATGATTCACAG
1B1	A133S mutation
	Forward CGGCGGCCGCAGCATGTCTTTCGGCCACTACTC
	Reverse GAGTAGTGGCCGAAAGACATGCTGCGGCCGCCG