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. 2016 Feb 5;17(2):214. doi: 10.3390/ijms17020214

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Inhibition of VEGF-induced cell migration. (A) Inhibition of VEGF-induced VEGFR2 phosphorylation. HUVECs were starved and treated with VEGF and test antibodies. Cell lysates were electrophoresed and analyzed by Western blot with anti-VEGFR2 and anti-phospho-VEGFR2 (Tyr1175) antibody; (B) The migration activity of HUVECs was evaluated using a Transwell assay system. Cells were added onto the top chamber, whereas migration stimuli VEGF mixed with various concentrations of the test antibodies were added to the lower chamber. After 22 h of incubation, the cells on the lower face of the membrane were stained and counted under microscope at 100× magnification; (C) The cell counts were obtained from three random fields and presented as mean ± SD.