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. 2016 Feb 8;17(2):227. doi: 10.3390/ijms17020227

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunohistochemical analysis of nuclear p(phospho)-Smad2/3 (brown nuclei) with methyl green counter staining (green nuclei). Original magnifications: ×200 (A); The positivity of each antigen in the muscle fibers was expressed as a distribution of the percentage of the total 1500 myofibers analyzed on the ×400 field. Data is shown as mean ± SD (** p < 0.01). Immunoblotting of p-Smad2/3 (B). The graph represents the relative band density to β-tubulin. Data is shown as mean ± SD (** p < 0.01).