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. 2016 Feb 10;17(2):231. doi: 10.3390/ijms17020231

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of SelR gene knockdown on d-galactose-induced cytochrome c release. (a) Purity of cytosolic and mitochondrial fractions; (b) ELISA quantization of cytochrome c protein in cytosolic and mitochondrial fractions. The data are expressed as the ratio of cytosolic-to- mitochondrial cytochrome c concentrations. * p < 0.05, *** p < 0.001, compared to the negative control group. NC: negative control cells; Si: cells with SelR siRNA transfection for 24 h; G: cells exposed to d-galactose (150 mM) for 36 h; Si+G: cells with SelR siRNA transfection followed by d-galactose exposure.