Figure 2.

Changes in glucocorticoid receptor and myogenic mRNA expression during myotube formation. C₂C₁₂ myoblasts were induced to differentiate into myotubes starting at ~90% confluence, d0 (day zero). Differentiation was carried out for four days, d1 (day one) through d4 (day four). (A) GRβ. # p ≤ 0.0009 compared to d0; ^$ p < 0.05 compared to d1; (B) GRα. ^# p < 0.01 compared to d0; ^$ p < 0.01 compared to d1; (C) MyoD. ^# p = 0.0179 compared to d0; (D) Myogenin. ^# p < 0.0001 compared to d0, ^$ p < 0.0001 compared to d1, and ^& p < 0.0001 compared to d2 (day two). n = 3 to 6 experiments per time point. Data expressed as mean relative quantification (RQ) ± SEM.