Figure 3.
GRβ overexpression reduces dexamethasone responsiveness in C2C12 myoblasts. (A) Myoblasts overexpressing GRβ (GRβOE) had a significant increase in GRβ mRNA expression compared to vector transfected cells. * p = 0.016; (B) GRα mRNA expression was not altered by GRβ overexpression; (C) GRβ mRNA expression was not altered in GRβOE cells following 2-h dexamethasone (Dex) treatment. **** p < 0.0001 as indicated by brackets; (D) Glucocorticoid-induced leucine zipper (GILZ) mRNA levels showed an abrogated response to Dex in GRβOE myoblasts. **** p < 0.0001 compared to respective Ctrl and as indicated by brackets. n = 3 to 5 experiments. Data expressed as mean RQ ± SEM.