Figure 2.

Effects of hinokitiol on cell viability and melanin production in B16F10 cell. B16F10 cells were treated with indicated concentrations of hinokitiol for 48 h. (a) Cell viability was measured by WST-1 assay; (b) Effect of hinokitiol on cellular melanin content; (c) Photograph of precipitated B16F10 melanoma cells. Cells were incubated for 48 h with (1.25–125 nM) and without hinokitiol; (d) Tyrosinase activity was measured. * p < 0.05 (mean ± SD, n = 6). Each experiment was repeated three times with similar results.