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The expression levels of tyrosinase and microphthalmia-associated transcription factor (MITF) after hinokitiol treatment. B16F10 cells were treated with hinokitiol at the concentration of 1.25, 12.5 or 125 nM for 48 h. The protein expression was determined by immunoblotting. Inserted values indicated relative proteins expression in comparison with β-actin. Each experiment was repeated three times with similar results. (mean ± SD, n = 3).
