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Hinokitiol reduces melanin content through AKT pathway. The B16F10 (105) cells were transfected with constitutively active AKT plasmid (5 μg) for 16 h prior to treated with hinokitiol (125 nM) for 48 h. The melanin expression were measured. * p < 0.05; *** p < 0.001 (mean ± SD, n = 6). Each experiment was repeated three times with similar results.
