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Constructed FELs of the proteinase K-solvent system at the four combined temperatures. (A–D) FELs as a function of two-dimensional conformational subspace at the combined temperatures: (A) P180/S180; (B) P300/S180; (C) P180/S300; and (D) P300/S300. The color bar represents the free energy value in unit of kJ/mol; (E,F) One-dimensional FELs along the projection of the eigenvectors (E) 1 and (F) 2. Black line: P180/S180; red line: P180/S300; green line: P300/S180; blue line: P300/S300.
