TABLE 1.
Characteristics of each GH43 subfamily
| Subfamily | Size of family (no. of sequences) | Taxonomic distribution | EC no. (no. of sequences)a | No. of characterized enzymes | Ligand(s)b | PDB code for 3D structure (1 per enzyme) |
|---|---|---|---|---|---|---|
| GH43c | 122 | 3.2.1.55 | 2 | |||
| GH43_1 | 254 | 3.2.1.37 (11), (3.2.1.37 + 3.2.1.55) (2) | 13 | BWX, pNP-aLAraf, pNP-BXYL | 4MLG | |
| GH43_2 | 31 | Neocallimastigomycota | 0 | |||
| GH43_3 | 87 | 0 | ||||
| GH43_4 | 264 | Neocallimastigomycota | 3.2.1.99 | 12 | SBAR, LAR | 2X8F, 3KMV, 3LV4, 4KC7, 4KCA |
| GH43_5 | 211 | 3.2.1.99 | 9 | SBAR, LAR | 1GYD, 1UV4, 1WL7, 3CU9, 4KCB | |
| GH43_6 | 38 | Fungi | 3.2.1.99 | 9 | SBAR, LAR | |
| GH43_7 | 13 | Bacteria | 0 | |||
| GH43_8 | 26 | Bacteria | 0 | |||
| GH43_9 | 137 | Chytridiomycota | 0 | |||
| GH43_10 | 376 | 3.2.1.37 (1), (3.2.1.37 + 3.2.1.55) (1), 3.2.1.55 (7) | 9 | AAX, WAX, SBAR, MU-xylose, pNP-aLAraf, pNP-BXYL | ||
| GH43_11 | 583 | Ascomycota | 3.2.1.37 (14), (3.2.1.37 + 3.2.1.55) (4) | 18 | OSX, xylobiose, linear 1,4-β-xylan, pNP-aLAraf, pNP-BXYL | 1YI7, 1YIF, 1YRZ, 2EXH, 3C2U |
| GH43_12 | 395 | Mollusca | 3.2.1.37 (1), (3.2.1.37 + 3.2.1.55) (3), 3.2.1.55 (5) | 9 | WAX, MU-xylose, pNP-aLAraf | |
| GH43_13 | 15 | Fungi | 0 | |||
| GH43_14 | 34 | Fungi | 3.2.1.37 | 1 | WAX, pNP-BXYL | |
| GH43_15 | 9 | Bacteria | 0 | |||
| GH43_16 | 184 | Neocallimastigomycota | (3.2.1.37 + 3.2.1.55) (1), 3.2.1.55 (6) | 7 | WAX, linear arabinoxylan, OSX, RAX, MU-aLAraf | 1W0N, 3C7E |
| GH43_17 | 53 | 0 | 5C0P | |||
| GH43_18 | 61 | 0 | ||||
| GH43_19 | 53 | Neocallimastigomycota | 3.2.1.55 | 1 | ||
| GH43_20 | 19 | Basidiomycota | 0 | |||
| GH43_21 | 19 | Fungi | 3.2.1.55 | 1 | SBAR, pNP-aLAraf | |
| GH43_22 | 118 | 3.2.1.37 | 1 | Xylan oligosaccharide (unspecified source) | ||
| GH43_23 | 36 | 0 | ||||
| GH43_24 | 228 | 3.2.1.145 | 8 | LWG, 1,3-β-galactan | 3NQH, 3VSF | |
| GH43_25 | 9 | Fungi | 0 | |||
| GH43_26 | 851 | 3.2.1.55 | 3 | pNP-aLAraf | 3AKF, 3CPN | |
| GH43_27 | 32 | Bacteria | 3.2.1.37 (1), 3.2.1.55 (1) | 2 | OSX, pNP-BXYL, pNP-aLAraf | |
| GH43_28 | 75 | 0 | ||||
| GH43_29 | 218 | (3.2.1.37 + 3.2.1.55) (2), 3.2.1.55 (1), 3.2.1.8 (2) | 5 | pNP-BXYL, pNP-aLAraf | 3QED, 4NOV | |
| GH43_30 | 111 | 0 | ||||
| GH43_31 | 54 | Bacteroidetes | 0 | 3KST | ||
| GH43_32 | 32 | Rotifera | 0 | |||
| GH43_33 | 25 | 3.2.1.55 | 1 | pNP-aLAraf | 4QQS | |
| GH43_34 | 185 | 0 | 3QZ4 | |||
| GH43_35 | 63 | Neocallimastigomycota | (3.2.1.37 + 3.2.1.55) | 3 | pNP-BXYL, pNP-aLAraf | |
| GH43_36 | 47 | 3.2.1.55 | 2 | No substrates specified | 3ZXJ | |
| GH43_37 | 13 | 0 |
a
EC number for characterized enzymes (multiple EC numbers in parentheses belong to the same enzyme).
b
BWX, birchwood xylan; AAX, AZCL-arabinoxylan; MU, methylumbelliferyl; BXYL, beta-xylan (unspecified source); AAR, alpha-arabinan; WAX, wheat arabinoxylan; SBAR, sugar beet arabinan; OSX, oat spelt xylan; RAX, rye arabinoxylan; LWG, larchwood galactan; LAR, linear arabinan. Arabinan (sugar beet) corresponds to any of the following: linear 1,5-α-l-arabinan, debranched arabinan, linear arabinan, or red debranched arabinan.
c
Sequences that could not be assigned to a subfamily.