FIG 2.

Growth of HI variant expressing tdTomato. (A to C) BbHIpMQ was grown in PYE medium at different temperatures. Cell growth was measured by the change in culture turbidity (A), fluorescence (B), and cell enumeration (C). Each experiment was conducted in triplicate, with each value representing the mean and standard deviation. (D, E) BbHIpMQ cells were cultured in a 96-well plate and grown in a Synergy H1 microplate reader at 30°C. Growth was measured by the change in culture turbidity (D) and fluorescence (E). Each value represents the mean and standard deviation of 8 wells.