FIG 2.
Growth of a selection of yeast strains sensitive to iron in media with high iron concentrations. (A) The indicated S. cerevisiae strains were grown overnight in liquid SC medium and then spotted in 1:10 dilutions, starting at an OD600 of 0.1 on solid SC medium plates that contained increasing FeCl3 concentrations. Given that HCl was used to prepare iron stock solutions, a control with the equivalent final HCl concentration was always used. Plates were incubated at 30°C for 3 days and then photographed. (B) After overnight growth in liquid SC medium, yeast cells were reinoculated at an OD600 of 0.2 and incubated at 30°C with agitation in the liquid SC supplemented with the indicated FAS concentrations. The number of cells per milliliter after 24 h of incubation is presented. Data and error bars represent the averages and standard deviations of the results from at least three independent biological samples.