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RT-PCR analysis of ndhAB and pno transcription in A. tumefaciens S33. Lanes 1 and 2, cDNAs synthesized from total RNAs isolated from cells cultured in nicotine medium and in glucose and ammonium medium, respectively; lane 3, negative control (total RNA isolated from cells cultured in nicotine medium); lane 4, positive control (genomic DNA from the culture grown in glucose and ammonium medium).
