TABLE 1.

Candidate genes involved in MZ1T flocculation^a

% SNP	Gene	Gene product function	Type of mutation	Base change (NextGENe software)	Amino acid change	Coverage	Mutant strain
100	tmz1t_3249	Polysaccharide deacetylase	Nonsense	G to A	W111 stop	12×	20A
90	tmz1t_3637	Family 2 glycosyltransferase	Frameshift	G/−d	V162Ab	15×	20A
93	tmz1t_3801	Polysaccharide biosynthesis CapD	Nonsynonymous	T to G	L102Rc	29×	20A
100	tmz1t_0834	Type IV pilus assembly protein	Nonsynonymous	G to A	A36Tb	17×	39A
100	tmz1t_1376	ABC transporter	Nonsynonymous	G to A	G170Rc	19×	39A
70	tmz1t_1383	Von Willebrand factor	Nonsynonymous	G to A	V162Ab	17×	39A
100	tmz1t_1679	PAS/PAC sensor containing diguanylate cyclase	Nonsynonymous	G to A	T615Ib	14×	39A
100	tmz1t_2095	CzcA family heavy-metal efflux pump	Nonsynonymous	A to T	V57Ec	17×	39A
90	tmz1t_3143	Diguanylate cyclase	Frameshift	C/−d	L508Wb	11×	39A
100	tmz1t_3810	Glucose-1-phosphate thymidylyltransferase	Nonsynonymous	G to A	R128Hb	22×	39A

^aThe table shows loci with %SNP values of >75% and coverage of >10×. The percentage of the single most prevalent nonreference base in the aligned column is also shown.

^bAmino acid change predicted to result in mild property change.

^cAmino acid change predicted to result in severe property change.

^dBase deletion.