Detrended correspondence analysis for samples on the basis of read abundances. The read numbers were log10(n + 1) transformed. Only a selection of the significant environmental parameters (vectors) (see Table S5 in the supplemental material) is shown. Overlapping clusters show the 0.95 confidence limits of the season grouping. Julian days 17, 68, and 110 were removed from the ordination analyses due to missing environmental data. The different seasons are color coded as shown in the key. o, samples from DNA libraries; +, samples from RNA libraries. C.N, C/N ratio.