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FISH visualization of 3-month-old biofilm material of BF2 sampled in February 2011, stained with genus-specific probes. (A) Section of a huge Nitrotoga colony stained with probe Ntoga-122. (B) Dense biofilm reveals a large number of Ntoga-122-stained Nitrotoga clusters (pink) and fewer Ntspa-712- and Ntspa-662-stained microcolonies of Nitrospira (green). EUB338-, EUB338II-, and EUB338III-stained cells are colored blue.
