TABLE 1.
Identification of NOB present in BF1 and BF2 by genus-specific PCR and TEM
| Biofilter and sampling date (mo/yr) | PCRa |
TEMb |
||||
|---|---|---|---|---|---|---|
| Nitrotoga | Nitrospira | Nitrobacter | Nitrotoga | Nitrospira | Nitrobacter | |
| BF1 | ||||||
| 07/2010 | + | + | – | +++ | ++ | – |
| 10/2010 | + | + | – | |||
| BF2 | ||||||
| 11/2010 | + | + | – | + | ++ | – |
| 12/2010 | + | + | – | |||
| 03/2011 | + | + | – | |||
| 06/2011 | – | + | – | + | ++ | – |
| 07/2011 | – | + | + | |||
| 08/2011 | + | + | + | |||
| 09/2011 | + | + | + | |||
a
+, positive reaction; −, no reaction.
b
+, present; −, not present. The number of plus signs reflects the approximate abundance of the respective NOB visible via TEM.