Table I.
Primer sequences used for polymerase chain reactions
| Gene | Primers | Temperature (°C) |
|---|---|---|
| Bsp | Forward: 5′‐AAAGTGAAGGAAAGCGACGA‐3′ | 58 |
| Reversed: 5′‐GTTCCTTCTGCACCTGCTTC‐3′ | ||
| Col1a1 | Forward: 5′‐CCTGACGCATGGCCAAGAAGA‐3′ | 60 |
| Reversed: 5′‐GCATTGCACGTCATCGCACA‐3′ | ||
| CREB‐2 | Forward: 5′‐GAAACCTCATGGGTTCTCCA‐3′ | 58 |
| Reversed: 5′‐AGAGCTCATCTGGCATGGTT‐3′ | ||
| Cyclo A | Forward: 5′‐GAGCTCTGAGCACTGGAGAGA‐3′ | 64 |
| Reversed: 5′‐GATGCCAGGACCTGTATGCT‐3′ | ||
| Dlx3 | Forward: 5′‐GCGACACTCAGGAATCATTG‐3′ | 55 |
| Reversed: 5′‐CGGTCCATGCATTTGTTATC‐3′ | ||
| Dmp1 | Forward: 5′‐CAGTGAGGATGAGGCAGACA‐3′ | 62 |
| Reversed: 5′‐TCGATCGCTCCTGGTACTCT‐3′ | ||
| Mmp‐2 | Forward: 5′‐CATCGCCCATCATCAAGTTCC‐3′ | 62 |
| Reversed: 5′CCGAGCAAAAGCATCATCCAC‐3′ | ||
| Mmp‐9 | Forward: 5′‐TGGTGTGCCCTGGAACTCA‐3′ | 64 |
| Reversed: 5′‐TGGAAACTCACACGCCAGAAG‐3′ | ||
| Oc | Forward: 5′‐CTTGGTGCACACCTAGCAGA‐3′ | 58 |
| Reversed: 5′‐TTCTGTTTCCTCCCTGCTGT‐3′ | ||
| Opn | Forward: 5′‐TCTGATGAGACCGTCACTGC‐3′ | 54 |
| Reversed: 5′‐AGGTCCTCATCTGTGGCATC‐3′ | ||
| Osn | Forward: 5′‐AAACATGGCAAGGTGTGTGA‐3′ | 54 |
| Reversed: 5′‐TTGCATGGTCCGATGTAGTC‐3′ | ||
| Osx | Forward: 5′‐ACTCATCCCTATGGCTCGTG‐3′ | 55 |
| Reversed: 5′‐GGTAGGGAGCTGGGTTAAGG‐3′ | ||
| Runx2 | Forward: 5’‐TACAAACCATACCCAGTCCCTGTTT ‐3’ | 66 |
| Reversed: 5′‐AGTGCTCTAACCACAGTCCATGCA ‐3′ | ||
| Floxed BMP2 | Forward: 5′‐GATGATGAGGTTCTTGGCGG‐3′ | 64 |
| Reversed: 5′‐AGGGTTTCAGGTCAGTTTCCG‐3′ | ||
| Exon3 BMP2 | Forward: 5′‐CGGGAACAGATACAGGAAGC‐3′ | 58 |
| Reversed: 5′‐GCTGTTTGTGTTTGGCTTGA‐3′ | ||
| Recom BMP2 | Forward: 5′‐GATGATGAGGTTCTTGGCGG‐3′ | 64 |
| Reversed: 5′‐AGCATGAACCCTCATGTGTTGG‐3’ | ||
| Floxed BMP4 | Forward: 5′‐AGACTCTTTAGTGAGCATTTTCAAC‐3′ | 55 |
| Reversed: 5′‐AGCCCAATTTCCACAACTTC‐3′ | ||
| Exon4 BMP4 | Forward: 5′‐TTCCTGGACACCTCATCACA‐3′ | 58 |
| Reversed: 5′‐CCACTCCCTTGAGGTAACGA‐3′ | ||
| Recom BMP4 | Forward: 5′‐AGACTCTTTAGTGAGCATTTTCAAC‐3′ | 64 |
| Reversed: 5′‐AGGTGAGCAGAGCTAAGATG‐3′ |
Bsp, bone sialoprotein; Col1α1, alpha 1 collagen type; CREB‐2, cAMP responsive element binding protein 2; Cyclo A, Cyclophilin A; Dlx3, Distal‐less 3; Dmp1, dentin matrix protein 1; Mmp‐2, matrix metalloproteinase‐2; Mmp‐9, matrix metalloproteinase‐9; Oc, osteocalcin; Opn, osteopontin; Osn, osteonectin; Osx, Osterix; Recom, recombinant.