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. Author manuscript; available in PMC: 2016 Mar 9.
Published in final edited form as: Arthritis Rheumatol. 2015 May;67(5):1335–1344. doi: 10.1002/art.39062

Figure 4.

Figure 4

Effect of bosentan (BOS) on vascular abnormalities in endothelial cell–specific Fli-1–knockout (Fli-1 ECKO) mice. A, Wild-type (WT) mice and Fli-1 ECKO mice were injected intraperitoneally with bosentan or phosphate buffered saline (PBS) for 4 weeks. Levels of Fli-1 protein in dermal microvascular endothelial cells were evaluated by immunohistochemistry. B, Evans blue dye was injected into the tail vein, and the mice were killed after 30 minutes. The leakage of Evans blue dye (arrows) was macroscopically evaluated in the skin. C, Levels of α-smooth muscle actin (α-SMA) were determined by immunohistochemistry. D, Mice were injected with fluorescein isothiocyanate (FITC)–conjugated dextran and killed after 5 minutes. The structure of dermal small vessels was examined by fluorescence microscopy. Results are representative of 5 independent experiments.