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. 2016 Mar 8;16:26. doi: 10.1186/s12886-016-0202-8

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© Yamada et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Gelatin zymogram showing the band densities of MMP-2 and MMP-9. Only bands of activeMMP-2 were detected after treatment with the prostaglandin analogs. No bands were detected without treatment (control lane). Samples containing 20 μg of protein were analyzed. B: bimatoprost, L: latanoprost, T: tafluprost. C: control, M: molecular weight marker. Molecular weights of proMMP-9, proMMP-2, and activeMMP-2 are 92 kDa, 72 kDa, and 62 kDa, respectively