Table 1.

Verification, validation, and diagnostic evaluation of the highest ranking top 15 genes

Rank	Gene	Optimized	Verification	Validation	1st diagnostic evaluation	2nd diagnostic evaluation
1	ZSCAN1	Yes	Yes	Yes	Yes	Yes
2	PCDH17	Yes	Noa
3	ST6GALNAC5	Yes	Yes	Yes	Yes	Yes
4	CLIC6	Yes	No
5	RP11-89 K21.1.1	Yes	No
6	ANKRD18CP	Yes	Yes	Yes	Yes	Yes
7	PAX2 b	Yes	Yes	Yes	No
8	CDH6	Yes	Yes	Yes	Yes	Yes
9	GFRA1	Yes	Yes	Yes	Yes	Yes
10	IRX1	Yes	No
11	POU4F3	Yes	Yes	Noa
12	GATA4	Yes	Yes	Yes	Yes	Yes
13	MKX	No
14	PAX2 b	Yes	No
15	KCNIP4	Yes	Yes	Yes	Yes	Yes
16	LHX8	Yes	Yes	Yes	Yes	Yes

^aExcluded due to high methylation in leukocytes

^bSame gene, different region