Table 2.
Methylation positivity in an external cohort of FFPE samples to validate results of high methylation in CIN2+ lesions and no methylation in normal cervices of the newly found methylation markers
| Gene | Normal | CIN2 | CIN3 | adCIS | Carcinoma |
|---|---|---|---|---|---|
| ZSCAN1 | 4/16 | 8/8 | 7/8 | 3/3 | 12/13 |
| ST6GALNAC5 | 0/16 | 1/6 | 4/8 | 2/3 | 9/12 |
| ANKRD18CP | 0/16 | 1/8 | 1/7 | 2/3 | 6/12 |
| PAX2 | 1/14 | 6/8 | 7/8 | 3/3 | 5/13 |
| CDH6 | 1/15 | 3/8 | 4/8 | 3/3 | 7/13 |
| GFRA1 | 0/12 | 2/8 | 3/8 | 2/3 | 10/12 |
| POU4F3 a | 2/14 | 6/7 | 3/7 | 3/3 | 11/12 |
| GATA4 | 0/17 | 3/8 | 2/7 | 3/3 | 10/13 |
| KCNIP4 | 0/17 | 6/8 | 5/8 | 3/3 | 10/12 |
| LHX8 | 1/16 | 3/8 | 4/8 | 3/3 | 7/13 |
aExcluded due to high methylation in leukocytes