Fig. 1.

Inhibitory influences of PEE on UBUC cells. a T24 cell proliferation assay. T24 cells were incubated with 50 μg/mL PEE for the indicated times and cell proliferation was measured by cell counting assay as described in an additional file [Additional file 1]. The diagram was the typical result of three independent experiments. All the data were expressed as mean ± standard deviation (S.D.) of the mean of four wells. b T24 cell migration. T24 cells were incubated with 50 μg/mL of PEE for the indicated times and cell migration was observed as described in an additional file [Additional file 1]. The results were expressed as mean ± S.D. of the mean of three independent experiments. c J82 cell proliferation assay. d J82 cell migration. e TSGH8301 cell survival assay. f T24 cell survival assay for proteomics study. T24 cells were treated with 50 μg/mL PEE for the indicated durations and cell viability was measured by MTT assay as described in an additional file [Additional file 1]. 0.5 % (v/v) DMSO-treated cells were recognized as the control in all the assays. TSGH8301 cell was incubated with the indicated doses of PEE for 72 h and cell viability was measured by MTT assay. 0 μg/ml indicated DMSO control. * and ** represented P < 0.05 and P < 0.001 respectively as compared to untreated cell using Student’s t-test