Table 1.
Differentially expressed proteins identified by tandem mass spectrometrya
| Spot | Protein identity | Incidences | Experiment pI/MW (kDa) | Theoretical pI/MW (kDa) | Matchedb peptide number | Coverage (%) | Accession number (NCBI) | Fold |
|---|---|---|---|---|---|---|---|---|
| 1 | ubiquilin 1 (UBQLN1) | 5/9 | 4.8/56.2 | 5.2/62.5 | 10 | 10.34 | 119583063 | +2.73 |
| 2 | DNA polymerase ε subunit 3 (POLE3) | 5/9 | 4.7/20.2 | 4.68/16.86 | 6 | 32.65 | 119607787 | +7.58 |
| 3 | eukaryotic translation initiation factor 5A (eIF5A) | 6/9 | 5/19.6 | 5.07/16.83 | 4 | 36.36 | 119610625 | +1.78 |
| 4 | tumor protein D54 (TPD52L2) | 6/9 | 4.9/29.9 | 5.26/22.24 | 8 | 44.66 | 11125673 | +12.84 |
| 5 | phosphohistidine phosphatase 1 (PHPT1) | 6/9 | 5.3/17.6 | 5.65/13.83 | 2 | 22.4 | 11191302 | +5.12 |
| 6 | proteasome inhibitor subunit 1 (PSMF1/PI31) | 5/9 | 5.1/32.3 | 5.42/29.82 | 6 | 25.83 | 10432572 | +3.97 |
| 7 | B-cell lymphoma/leukemia 10 (BCL 10) | 5/9 | 5.3/31.8 | 5.57/26.25 | 3 | 12.02 | 119593614 | +2.9 |
| 8 | dUTP pyrophosphatase (DUT) | 5/9 | 5.5/21.2 | 6.15/17.75 | 5 | 42.07 | 10257385 | +6.81 |
| 9 | prefoldin subunit 5 (PFDN5) | 5/9 | 5.8/19 | 5.94/17.33 | 9 | 57.79 | 119617089 | +2.51 |
| 10 | peflin (PEF1) | 7/9 | 5.8/30.8 | 4.84/28.22 | 7 | 23.6 | 119628003 | +6.31 |
| 11 | triosephosphate isomerase 1 (TPI1) | 5/9 | 6.2/28 | 5.65/30.79 | 8 | 39.86 | 119609128 | +6.27 |
| 12 | 26S proteasome non-ATPase regulatory subunit 9 (PSMD9) | 5/9 | 6.3/30.5 | 6.46/24.68 | 2 | 9.42 | 119618700 | +7.17 |
| 13 | NADH dehydrogenase (ubiquinone) 1 α subcomplex assembly factor 1 (NDUFAF1) | 5/9 | 6.3/34.9 | 7.11/37.76 | 4 | 17.43 | 119612893 | +5.2 |
| 14 | transgelin 2 (TAGLN2) | 5/9 | 6.9/22.6 | 8.41/22.39 | 10 | 53.27 | 119573145 | +3.2 |
| 15 | Diablo (DIABLO) | 5/9 | 4.8/21.5 | 5.67/27.16 | 10 | 43.51 | 10437144 | +3.94 |
| 16 | BCL2-associated athanogene 2 (BAG2) | 5/9 | 5.9/26.3 | 6.25/23.77 | 9 | 43.60 | 115528700 | +4.71 |
| 17 | translin-associated factor X (TSNAX) | 5/9 | 6.2/33.3 | 6.10/33.11 | 8 | 42.41 | 119590369 | +6.54 |
| 18 | profilin 1 (PFN1) | 5/9 | 6.8/17.9 | 8.44/15.05 | 7 | 65.00 | 119610787 | +1.57 |
| 19 | cofilin (CFL1) | 5/9 | 7.0/19.0 | 8.22/18.50 | 10 | 56.02 | 116848 | +2.86 |
| A | F-actin-capping protein subunit alpha-1 (CAPZA1) | 5/9 | 5.2/36.8 | 5.45/32.92 | 10 | 41.26 | 119576929 | −1.93 |
aMatched peptide number: Number of peptides matched with protein in MS/MS query. Coverage: Total percentage of amino acid sequence covered by peptides identified by MS/MS analyses
bThe detail data of MS/MS identification for each peptide was provided in Additional file 2: Table S2, supporting information