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. 2016 Mar 9;16:17. doi: 10.1186/s12935-016-0294-5

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© Lin et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Effect of C/EBPα on the expression of let-7a-1. a Detection of ectopic C/EBPα overexpression in H1299 cells and knockdown in Hela cells by western blotting. For each sample, 30 μg total proteins were used. Detection of (b) induction of pri-let-7a-1 by ectopic C/EBPα expression in H1299 cells, and (c) reduction of pri-let-7a-1 by C/EBPα knockdown in Hela cells. Quantitative real-time polymerase chain reaction was performed to detect the level of pri-let-7a-1. Data are represented as means ± standard deviations from three independent tests. *P < 0.05. Detection of (d) let-7a induction by ectopic C/EBPα expression in H1299 cells, and (e) let-7a reduction by ectopic c/EBPα knockdown in Hela cells by Dual-luciferase reporter gene assay. Data are represented as means ± standard deviations from three independent tests. *P < 0.05