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. 2016 Mar 9;16:17. doi: 10.1186/s12935-016-0294-5

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© Lin et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Targeting of let-7a-1 promoter by C/EBPα. ChIP assay was performed on H1299 cells transfeted with pcDNA3.1(+) or pcDNA3.1(+)-C/EBPα. C/EBPα proteins were immunoprecipitated from DNA–protein cross-linked cell lysates. After the removal of all proteins, the recovered DNA was precipitated by ethanol and amplified by PCR using primers specific for let-7a-1 promoter