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. Author manuscript; available in PMC: 2016 Mar 9.
Published in final edited form as: Cell Signal. 2013 Mar 14;25(6):1339–1347. doi: 10.1016/j.cellsig.2013.02.022

Fig. 3.

Fig. 3

TTP-deficient BMMCs develop and degranulate comparable to WT BMMCs. (A) Differentiation of WT and TTP-deficient BMMCs after 5 weeks in culture was determined by FACS analysis measuring surface expression of FcεRI and c-Kit. Comparable results were obtained in 5 independent WT and TTP-deficient BMMC cultures. (B) WT (■) and TTP-deficient (KO) BMMCs (□) were preloaded with IgE overnight. Cells were left untreated (con) or stimulated with 20 ng/ml antigen (DNP-HSA) for 20 min. Degranulation was assessed by relative release of β-hexosaminidase. Each bar represents the mean ± SD of three independent BMMC cultures, each taken from the mean of duplicates.