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. Author manuscript; available in PMC: 2016 Mar 9.
Published in final edited form as: Cell Signal. 2013 Mar 14;25(6):1339–1347. doi: 10.1016/j.cellsig.2013.02.022

Fig. 7.

Fig. 7

Other TIS11 family proteins as well as the ARE-mRNA stabilizing protein Zfand5 are expressed in BMMCs. (A) WT (■) and TTP-deficient BMMCs (□) were left untreated (con) or stimulated with 3 μg/ml LPS for 90 min or 3 h. BRF1 (top panel), BRF2 (middle panel) and Zfand5 mRNA (bottom panel) was analyzed by RT-qPCR. Each bar represents the mean ± SD of three independent BMMC cultures. Comparable results were obtained in several experiments (n ≥ 3). (B) WT BMMCs were left untreated (con) or stimulated with 3 μg/ml LPS for 2 h. Pellets were lysed and PS were subjected to anti-14-3-3 immunoprecipitation. Precipitates (IP, left panels) as well as PS (right panels) were analyzed by anti-BRF1/2 (top panels) and anti-14-3-3 (bottom panels) immunoblotting. Densitometry was performed and relative expression levels (normalization on precipitated 14-3-3) are indicated.