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. Author manuscript; available in PMC: 2016 Mar 9.
Published in final edited form as: Cell Microbiol. 2013 Mar 22;15(8):1427–1437. doi: 10.1111/cmi.12130

Figure 3. Impact of PSM deletions on neutrophil lysis after phagocytosis.

Figure 3

(A) Analysis of neutrophil phagocytosis of FITC-labeled MW2 and isogenic agr, PSM, and PVL mutant strains. Neutrophils were allowed to phagocytose MW2 strains in the presence of human serum. After 30 min phagocytosis was measured by flow cytometry. (B) Neutrophil lysis 3hrs after phagocytosis (MOI 10) of MW2 strains, as determined by LDH release. Data represent the mean ± SEM of 4–5 independent experiments with neutrophils isolated from different donors.